Reporter
Part:BBa_K2017006:Design
Designed by: Monica Victoria Gutierrez Salazar Group: iGEM16_Valencia_UPV (2016-10-03)
RSIAT-TEV linker + Luciferase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 855
Design Notes
This part must be fused downstream to other coding sequence. In order to be used in our modular gRNA testing system, it was necessary to remove the ATG (Met codon) to avoid unexpected translation. The translation must begin in the coding sequence fused upstream. The linker includes a random nucleotide in 5', to change the reading frame of the luciferase. That way, it will not be translated unless an indel is produced in the coding region to which the part is fused.
Source
Luciferase was obtained from laboratory stock. The linker was fused with a PCR.